Welcome to the Salomon Research Group

Welcome to the Salomon Laboratory

We uncover how signaling networks control cellular responses and communication.

The Salomon Laboratory combines mechanistic biochemistry with quantitative phosphoproteomics to discover hidden feedback, pathway crosstalk, and unexpected signaling consequences in T cells, CAR T cells, and cancer-relevant target cells.

  • How are signals controlled?

    We define feedback, phosphatase control, and pathway crosstalk that set immune-cell activation thresholds.

  • What changes during cell contact?

    We resolve signaling from each cell type within mixed cocultures, revealing responses obscured when their signals are analyzed together.

  • Which mechanisms matter?

    We connect quantitative phosphorylation networks to feedback control, pathway crosstalk, and signaling outcomes.

Explore our research
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Latest published discovery Science Signaling

CAR target cells are active participants

Most CAR studies ask what the engineered T cell does. Our recent study asks what happens inside the contacted target cell. Cell-of-origin phosphotyrosine proteomics shows that a CSF1R-directed CAR triggers CSF1R-like signaling in THP-1 target cells, whereas CD19-CAR contact does not produce the same signaling response in Raji targets.

  • The target signals: CSF1R Tyr723 and downstream ERK phosphorylation increase after CSF1R-CAR contact.
  • The mechanism is target-cell intrinsic: the response requires CSF1R kinase activity but not T-cell Lck activity or actin polymerization.
  • Cell ratio changes the outcome: target-rich conditions promote THP-1 growth, whereas effector-rich conditions produce target-cell lysis.

Read the Science Signaling paper (opens in a new tab)
View in PubMed (opens in a new tab)

Diagram comparing CSF1R-CAR target-cell outcomes across effector-to-target ratios. A complete text description follows.
Conceptual summary of the published result. CSF1R-CAR T cells promote THP-1 target-cell growth at low effector-to-target ratios and target-cell lysis at high ratios.
Text description of CAR target-cell diagram

Overall structure: The diagram reads from left to right as the number of CSF1R-CAR T cells per target cell increases. Rose circles represent CAR T cells, gold circles represent CSF1R-positive target cells, and an arrow along the bottom shows the increasing effector-to-target ratio.

Target-rich contact, low effector-to-target ratio
A CAR T cell contacts a target cell and activates CSF1R phosphotyrosine signaling. Under these target-rich conditions, the observed outcome is target-cell growth.
The target is not passive
Cell-of-origin phosphoproteomics separates signals from the two cell types. Target-cell signaling requires the kinase activity of CSF1R. T-cell Lck activity and actin polymerization are not required for this response.
Effector-rich contact, high effector-to-target ratio
Multiple CAR T cells contact a target cell. Under these effector-rich conditions, the observed outcome shifts to target-cell lysis.

CSF1R Low Ratio

A musical take on the target-cell response.

Music created with Suno.com.